|
KMID : 0624620100430090604
|
|
BMB Reports
2010 Volume.43 No. 9 p.604 ~ p.608
|
|
|
Inhibitory effects of antithrombin on the expression of secretory group IIA phospholipase A2 in endothelial cells
|
|
Kim Tae-Hoon
Bae Jong-Sup
|
|
|
Abstract
|
|
|
|
Tumor necrosis factor-α (TNF-α) mediates proinflammatory responses in primary human umbilical vein endothelial cells (HUVECs), and it upregulates the expression of secretory group IIA phospholipase A(2) (sPLA(2)-IIA). sPLA(2)-IIA plays a pivotal role in inflammation, and antithrombin (AT) possesses properties that are beneficial to endothelial cells. Therefore, we investigated the effects of AT on the expression of sPLA(2)-IIA in TNF-α-stimulated HUVECs. TNF-α potently upregulated the expression of sPLA(2)-IIA, and prior treatment of cells with AT inhibited the expression of sPLA(2)-IIA in HUVECs. Also, antibodies or siRNA for syndecan-4 blocked the protective effect of AT. Furthermore, PI3-kinase and the AKT pathway are significantly involved in the AT-mediated inhibition of the expression of sPLA(2)-IIA. These results show that AT effectively suppresses the upregulated sPLA(2)-IIA expression, which might contribute to the cytoprotective effects of AT in the treatment of severe inflammatory diseases.
|
|
|
KEYWORD
|
|
|
Antithrombin, HUVEC, Inflammation, sPLA2-IIA, TNF-α
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|